FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_B10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_B10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630910
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG60270.3695482889920351No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA43620.2674580448951812No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG42630.261387814165098No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC39440.24182818181260768No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA38920.238639777792766No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT33520.20552942835594853No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT33420.2049162737367482No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG32360.19841683477322478No Hit
GGGTAGTAGAGGGTAGATGCAGTCCAGCTAGCTGTCTGCCACTGGGCTGG32280.19792631107786451No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA31600.1937568596673023No Hit
CTTTCAGGTAGGGTAGTAGAGGGTAGATGCAGTCCAGCTAGCTGTCTGCC30410.18646031969881846No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT29660.18186166005481602No Hit
ACCTACAAGCGATATACAAGAAAGAGATCTAAACACAAAGACATAGGAGA29630.1816777136690559No Hit
ATATACAAGAAAGAGATCTAAACACAAAGACATAGGAGAATAAAAGTAAA28840.17683379217737338No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT28630.17554616747705268No Hit
GCTTTAAGGATTCTTTGGTGTTTGTTGAGACTTATGTTGTGGTCTGATGT26620.1632217596311262No Hit
AAATAAAGCCTTACCTACAAGCGATATACAAGAAAGAGATCTAAACACAA26490.16242465862616576No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT25860.15856178452520373No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG25700.1575807371344832No Hit
GACATAGGAGAATAAAAGTAAATGGAGGAGGAAACATAGTCGGCCAACCT25560.15672232066760275No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC25470.15617048151032245No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC24620.1509586672471197No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT24020.14727973953191775No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT23960.14691184676039756No Hit
TTTCTATCCCATCATTTTGCTGGCTTTGTCTTTGCTTGTTTTTTTCTTTT23660.1450723829027966No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG23410.1435394963547958No Hit
ATACAAGAAAGAGATCTAAACACAAAGACATAGGAGAATAAAAGTAAATG22900.14041240779687414No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT21450.13152166581846944No Hit
TTATTAAGCTTGATCCATGAATATATTTCAAACACTGCTCCTAAGAACTT21210.13005009473238865No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT21080.12925299372742824No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA20430.12526748870262613No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC20240.12410249492614553No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA19390.11889068066294277No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT19360.11870673427718267No Hit
CCCTTACTCATTCTCCATTGCTTTAAGGATTCTTTGGTGTTTGTTGAGAC19310.11840015696758252No Hit
AAAGCAGGGTGGGGTCTCAAAGCTCTTGGGCCCAGTGGACCACAGCACCA19210.1177870023483822No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA19150.117419109576862No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA19010.11656069310998156No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC18960.11625411580038138No Hit
GATATACGCATGTGCAAGTCTTAGCTTGTAAAATAACACCCCACACACTC18500.11343360455205989No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT18470.1132496581662998No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC18460.11318834270437976No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC18260.11196203346597912No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG18210.11165545615637895No Hit
GGGTAGATGCAGTCCAGCTAGCTGTCTGCCACTGGGCTGGTGGGAGACTC18180.11147150977061884No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA18170.11141019430869882No Hit
GTAGTAGAGGGTAGATGCAGTCCAGCTAGCTGTCTGCCACTGGGCTGGTG17970.11018388507029818No Hit
ATATAAAACCGAGAGTGAAATTTAGAAATCCAAGCCCAAAGAGGACTGGA17730.1087123139842174No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT17140.10509470173093549No Hit
CCTCTGGGTCACTTTCAGGTAGGGTAGTAGAGGGTAGATGCAGTCCAGCT16900.10362313064485472No Hit
CACAAAGACATAGGAGAATAAAAGTAAATGGAGGAGGAAACATAGTCGGC16870.10343918425909462No Hit
GTTCTTAGGAGCAGTGTTTGAAATATATTCATGGATCAAGCTTAATAACG16700.10239682140645408No Hit
CCCTTAAGATGTTAGTGTGATTTCTACCATAACAAAGACCCAGGTCAGTC16440.10080261939653323No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG16360.10031209570117296No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA800.0126.916381
AGCGAAA5700.0119.6003951
CAGGTAC1900.0103.0409553
GACGTAT406.119626E-790.623985145
GCAGGTA2400.087.61932
GCGAAAG8250.083.4992752
AGGTACT2550.082.462694
CGAAAGC8850.077.835923
GACGTAA350.003564899662.1516886
AGTAGAA15450.057.2678641
ATGCGTG400.006050367454.3777287
ACGTAAG400.006050367454.3777287
GAGCATA400.006051835654.374399
GGTGGTC553.2197832E-452.7266858
ACAAGGC7300.051.6432578
GGTCAAA9300.051.468391
GGTACTG4200.050.0666355
GTATTAG752.5491558E-548.3402023
ACTACTC752.5491558E-548.3402023
CTCGGGG450.00964064648.33279145
Produced by FastQC (version 0.11.5)