FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_B09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_B09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635841
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC1850.050.964523
AGCGAAA1850.047.051581
GCGAAAG1950.044.6351622
GTAGAAA5300.034.2159651
AGCTATC2551.8189894E-1234.1166659
CCGTGTA1551.4642246E-632.7409939
GTATACT900.00361798532.2390441
GTATATA1652.3749362E-630.773631
ACCGTGT1255.14788E-429.0037278
TAACGTG3301.8189894E-1228.5710185
TATAGTA2903.929017E-1027.5121922
ATTAACG3204.1836756E-1127.197413
ACCGTGC1907.142933E-626.713968
ATATAGG1100.0096555226.3711747
GTATTAT6100.026.161191
GCATTAA3151.02591E-925.3306751
AACGTGT3451.07320375E-1025.2265876
TTAACGT3601.8371793E-1024.1754784
ATAGTAC2802.331617E-723.3120673
GTATAGG1902.1842137E-422.9066891
Produced by FastQC (version 0.11.5)