FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_B04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_B04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127919
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC2790.21810677069082782No Hit
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC2030.1586941736567672No Hit
GGATAAGACTGAATGGAAACTGAACGGACAGGTGCTGGTCTTCACACTCC1530.11960693876593782No Hit
GTTAATGGGATGTCATCTACGCACACTGGGCTCAGCGCCTGGCACAGCGG1280.10006332132052313No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACC100.0070675323145.110721
TAGACGG100.0070758057145.053975
GCGATTA100.007084085144.99727145
ACCATCG301.5942549E-596.664848
CGAAAGC554.0324776E-665.9336243
TAAGGCT350.003554151462.1659855
CAAGGGT701.6838947E-551.804994
AGCGAAA752.5265803E-548.3702431
CCCTTAA604.910283E-448.370241
GCGAAAG752.5324352E-548.3513262
CCATCGG604.929273E-448.332429
TATATAG657.299115E-444.6319923
GACAGTG1703.8198777E-1142.646257
GTTTAGA700.001049234641.4602051
GTCTTAG700.001049234641.4602051
ATTAATT1001.3816456E-436.2634933
CGGCATC800.002030181236.249317145
GGACAGT1905.32782E-934.3548856
ATATAGA850.002728110634.1303444
CCTCGGC850.002733349634.1178
Produced by FastQC (version 0.11.5)