FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_B02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_B02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890579
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC9260.10397730016090656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3700.097.9946441
GGTACGG406.108876E-790.645041
CGAAAGC5300.068.3998263
CGGAGTA554.059968E-665.912575
GCGAAAG5500.065.912562
ACATCGA350.003566424862.1426438
CGGGTGT502.0096006E-458.003066
TACTAAC1402.728484E-1046.606988
TATTATC3050.045.163787
GCTATAC1351.0253643E-842.9652253
GGAGTAT855.339361E-542.6493036
GTACGGA907.4794574E-540.27992
GCGATTA750.001482915138.6687056
GATTACG750.001483324438.666538
ACTACTC1157.141998E-637.825968
GAGTCGC1854.1818566E-935.270159
TAATACC1051.8535917E-434.52369145
GGACAGT4250.034.1194426
ATACTAA2206.220944E-1032.954437
AGCGATT900.003627083532.223925
Produced by FastQC (version 0.11.5)