FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_B01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_B01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046171
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC24860.12149522205133394No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT21190.10355928219098012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAACG3600.048.3420756
GTAGAAA13700.041.8315121
CGAAAGC5300.039.6769832
GCGAAAG5850.035.9616131
AGCGAAA4450.030.9735221
GGACAGT23950.030.2768736
TAGGCCG2451.9754225E-929.591404145
TATAAGG9100.027.0928122
AAGTGAT18050.026.9135937
TCCCGAC1100.00966538126.3684062
AGTAGAA12950.025.7682611
AGTGATC19000.024.0408828
ACCGTGT7850.024.0141478
TAGAAAC22300.023.7374782
GACAGTG30950.022.9582887
CCGGAAT1650.002599050821.9736715
CCGTTAC1650.002602002821.9693749
GTATAAG13650.021.7895491
TATACGT4058.058123E-1021.4853674
ATAGCAC15250.021.3973123
Produced by FastQC (version 0.11.5)