FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_A12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_A12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832361
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACTGTGCTGCTTCTGAGAAACCAGCAGGGCCTGTTAATTCTTT47430.2588463736130599No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC40190.21933450886588396No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT30860.1684165947648962No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT30470.16628819321083563No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG27390.14947927837363925No Hit
GATAACATACTAACGTATGCTTCCTAGAATGAGAAGGAAGAAAATGAATA26940.14702343042664626No Hit
CCATAATAAATAGGAAACACTCGAGGTTCACTACAATTTGTCCTTATTCC26620.14527704966434016No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC25460.1389464194009805No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC24450.1334344051199518No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT20330.11094975280526054No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG19530.10658380089949525No Hit
GTCTGATATTCCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCG19510.1064746521018511No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3250.073.652441
TATACTG14100.067.375515
GCGAAAG3750.065.755662
ATATACT16950.052.196344
AATATAC17800.048.4829183
CGAAAGC5350.046.0879023
GCTATAT4150.043.696691
CGTTTAT5100.042.6685331
TACTGTG30500.041.1234477
ATACTGT24550.040.4686746
TAATATA22000.039.5588842
TATTATC8700.039.179972
GTAATAT22800.038.495251
ACCGTAC800.002032029736.2682531
AGATGTA11950.035.795417
GTATTAT11350.035.1498451
CATTAAG14550.033.8946652
GTATAGA6650.033.814011
AAGATGT12600.033.3816156
TATAGAC5850.032.2331662
Produced by FastQC (version 0.11.5)