FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_A11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_A11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650888
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACACATATTACTGGGTGTTTCCTTCATTTTAAAGAAATGCGAACA27270.4189660894040142No Hit
TTACAAAGGAGTACCATATGATGGAGACAGAACCATCTTGAGAAAATTGT20410.3135716129349443No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG19820.30450707341355193No Hit
GGTTACTTGTTGTTAGGAAGCAAAATCAAACTGATTACAATTACAAAGGA15210.23368075613623235No Hit
GAATAAGCCTGGCCTCAGTTTTTCTGACGCGACAGAGGTTTTGGGCACTC15060.23137621219011567No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC14830.2278425781394034No Hit
GTCATGAGGACAGACTGCAGGAGGCCTGGGACTACGTGCAGGCCCAGGTG14170.21770258477648996No Hit
ACTCTGGGTGGTCTGTTCATCATGGCTTCCTGCTGCTGCTTCATCCGGGT13700.21048168041199103No Hit
CCCCAAGAGATCGCCGCAGACTCCCATGACATCGAGTATATTGAAAAATA13120.20157077715367314No Hit
GTGTAATACAGAAGCATGAGTCAGGAGTAAATTTGTTCCCTGACACTCAT12590.1934280552107275No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA12370.1900480574230897No Hit
GTCTAAGGGAAAGCCGTGACCCCGGGGTCCAGCAGGTGCCTGCCCTGTGC12370.1900480574230897No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT12340.18958714863386636No Hit
CACTTATGTTCGCATTTCTTTAAAATGAAGGAAACACCCAGTAATATGTG12240.1880507860031219No Hit
GAAAAATACTGCGGGGTGGCTGTGGAAAGAGCCTTTGCTGGGGAAGCCAG11860.18221260800629294No Hit
GTTCAGGAGGACCCAGTTGTGCAGAAGCAGTGGGGGCCAGCTGGTGTCGC11720.1800617003232507No Hit
GGATAATCCCGCGCCACCTGCAACTCGCCATCCGCAACGACGAGGAGCTC10740.165005346541955No Hit
GGCCTGCAGTTCCCGGTGGGCCGAGTTCACAGACTGCTGCGCAAAGGTAA10670.16392989270043387No Hit
CTTATGTTCGCATTTCTTTAAAATGAAGGAAACACCCAGTAATATGTGTA10600.16285443885891274No Hit
CTCCCATGACATCGAGTATATTGAAAAATACTGCGGGGTGGCTGTGGAAA10410.1599353498604983No Hit
TTTCAAAGGTGGAGTAACTTTCCGTCTTGGAAGAGTGCTTCTGATGACAC10210.15686262459900935No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT10090.155018989442116No Hit
CTACAAGGACAGTCATGAGGACAGACTGCAGGAGGCCTGGGACTACGTGC9860.15148535539140376No Hit
GTGTTGGAGTACCTGACCGCCGAGATCCTGGAGTTGGCTGGCAATGCCGC9790.15040990154988262No Hit
CTTCTGTATTACACATATTACTGGGTGTTTCCTTCATTTTAAAGAAATGC9600.14749081255146815No Hit
ACCTCATTCAGAACTACAAGGACAGTCATGAGGACAGACTGCAGGAGGCC9590.1473371762883937No Hit
GTAATATGTGTAATACAGAAGCATGAGTCAGGAGTAAATTTGTTCCCTGA9420.14472535981612814No Hit
GATTACAATTACAAAGGAGTACCATATGATGGAGACAGAACCATCTTGAG9250.14211354334386253No Hit
CTCTAGAACTTTCTCATCTTGCAAAACTGAAATTCTGTAACTCCCCATGA9220.1416526345546392No Hit
ATATTTTTCTTAGCCCAATAGCCGTTGTGTTTTCAGGTTTTGGATATCAG9170.14088445323926696No Hit
CTCTACGGAAAGCTTACATTGCCTCTTGTGAGTGTATCCAATCTCTAGAA9110.1399626356608203No Hit
GTACCATATGATGGAGACAGAACCATCTTGAGAAAATTGTCTGAACGGCT8810.1353535477685869No Hit
GGGATGGAGAGAGGTGTTTGGCGGAGACGGAGGCGTGAGGTAGGGGCCCA8640.13274173129632133No Hit
ACTAAAGACACTGCACGGTTACGCAACATGAGTGTCAGGGAACAAATTTA8550.13135900492865132No Hit
CCTCAGTACTTGGGGACCTTGCTGTAGTCTTCAGAGTGGACGTGCCGGCA8550.13135900492865132No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG8550.13135900492865132No Hit
GCCTAGGATGATGCCCACGTTCTCCTGCAGCCAGGCCTGCACCTTCTTCA8310.1276717346148646No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA8300.12751809835179018No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA8260.12690355329949238No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA8230.12644264451026904No Hit
GTTACGCAACATGAGTGTCAGGGAACAAATTTACTCCTGACTCATGCTTC8210.12613537198412017No Hit
GTATACACACTTATGTTCGCATTTCTTTAAAATGAAGGAAACACCCAGTA8200.1259817357210457No Hit
TCTTCATGCAGCCCTCATGGTACACGGGCCAGTACTCGGGGCTGTTCCCG8160.12536719066874794No Hit
GCTCCTAAGCATGTAACTCATGACTAAAGACACTGCACGGTTACGCAACA8080.12413810056415236No Hit
ACATATTACTGGGTGTTTCCTTCATTTTAAAGAAATGCGAACATAAGTGT8040.12352355551185457No Hit
TACTTGTTGTTAGGAAGCAAAATCAAACTGATTACAATTACAAAGGAGTA8000.12290901045955678No Hit
AATCCATTCCTTCTGGGAAAAGCGGCTTTCAAATAAAACAGGCAAAAGAA7880.12106537530266344No Hit
ATATGATGGAGACAGAACCATCTTGAGAAAATTGTCTGAACGGCTCCTAA7870.12091173903958899No Hit
GTACTTGGGGACCTTGCTGTAGTCTTCAGAGTGGACGTGCCGGCACAAGC7850.1206044665134401No Hit
CATAGAGACAGAGAAATAGAATGATGGTTACCAGGGACTAAAGGGAGGTG7820.12014355772421675No Hit
TTGCTGTAGTCTTCAGAGTGGACGTGCCGGCACAAGCAGATGGACAGGAG7780.11952901267191897No Hit
CATAAGTGTGTATACAAAAATATCTGATATCCAAAACCTGAAAACACAAC7770.11937537640884453No Hit
GTACACGGGCCAGTACTCGGGGCTGTTCCCGCTCTCGGTCCTGTTGCTGT7740.1189144676196212No Hit
ACTTTGGTCAGCACCCTCGAGGTGTTTTGTGGGTGGGGCGAGGGAGACCA7710.11845355883039785No Hit
AAATCAAACTGATTACAATTACAAAGGAGTACCATATGATGGAGACAGAA7650.11753174125195118No Hit
CCCCAAGTGTTAACCAACAGATGATAAATCTTATGTACCATGGAGTATGT7580.11645628741043006No Hit
CATCGTGACCAACCTCATTCAGAACTACAAGGACAGTCATGAGGACAGAC7500.11522719730583449No Hit
GGCCAGTACTCGGGGCTGTTCCCGCTCTCGGTCCTGTTGCTGTCGAAAGC7430.11415174346431337No Hit
CTCCTGTCTTGTCTGGGCAGTTCAGGAGGACCCAGTTGTGCAGAAGCAGT7420.11399810720123893No Hit
CTAGAAGCCCATCTGTGGCATTCTTAAGATTTCATTTATCTGACAGAGAA7350.11292265335971781No Hit
TCTTGGAAGAGTGCTTCTGATGACACAGGGCCAACATATTTTTCTTAGCC7290.11200083578127114No Hit
ATGTAGTCTCGGGGACCTTCCGGATGACTCGGGGGGCAGCCAGGAGAGCT7190.11046447315052667No Hit
ACATAAGTGTGTATACAAAAATATCTGATATCCAAAACCTGAAAACACAA7190.11046447315052667No Hit
CTCTAGGCACCTTGTGTTAAGTAGAATCACAAAGTATTTCTTTTCGCAAC7140.10969629183515443No Hit
AAGCAGGCCTGCAAATCCATTCCTTCTGGGAAAAGCGGCTTTCAAATAAA7100.10908174678285665No Hit
AAGTAAGATATGCCAGTTGCGAAAAGAAATACTTTGTGATTCTACTTAAC7040.10815992920440998No Hit
CATATTACTGGGTGTTTCCTTCATTTTAAAGAAATGCGAACATAAGTGTG6990.10739174788903774No Hit
ATCCTGGAGTTGGCTGGCAATGCCGCTCGTGACAACAAGAAGACCAGGAT6940.10662356657366551No Hit
CTCCTGAACTGCCCAGACAAGACAGGAGAGTGAAGGCCCCGGGAATGACA6930.10646993031059107No Hit
GTGCGGGCCAAGGCCAAGTCTCGGTCCTCCCGCGCGGGCCTGCAGTTCCC6880.10570174899521884No Hit
CATATGGGTCACGAAATAGGTACATGGAATAAGCCTGGCCTCAGTTTTTC6870.1055481127321444No Hit
CATGAGGACAGACTGCAGGAGGCCTGGGACTACGTGCAGGCCCAGGTGAA6800.10447265889062328No Hit
CTTAATGACTGAGCCACTCAAGTGCCAACTGTGGCATACCTGTGAGAGCA6780.10416538636447438No Hit
GATGGAGACAGAACCATCTTGAGAAAATTGTCTGAACGGCTCCTAAGCAT6660.10232175120758102No Hit
GGCCTGCACCTTCTTCATGCAGCCCTCATGGTACACGGGCCAGTACTCGG6660.10232175120758102No Hit
CCATATGATGGAGACAGAACCATCTTGAGAAAATTGTCTGAACGGCTCCT6510.10001720726146433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5150.0124.022471
TAACGCC203.8659334E-4108.74654145
GAGCGAA700.0103.687621
GCGAAAG6700.095.294062
ACGTGTG259.380723E-487.0106057
GACGAGT353.42645E-582.9054646
GACGTGT300.001930966372.542286
AGGGACG300.001930966372.542283
ACGAGTG406.6574205E-572.5088357
TTATAGG451.1901077E-464.4820253
CGAGTGC350.00356530862.1456578
GGCTTCG350.003566393462.14088145
TCGCGTC2400.054.373272145
CAGTCCG400.006050702754.373279
GAAAGCA11750.054.3295364
GGTCAAC1353.6379788E-1253.763951
TACTTGT5050.053.1497884
GGAATCC1004.6982677E-850.7522858
TCACGAA3050.049.9164439
ATTATTG2050.049.5410653
Produced by FastQC (version 0.11.5)