FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_A10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_A10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200061
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC3900.044.6294633
AGCGAAA3950.042.2337951
CAATTCG4600.039.401479
GCGAAAG5050.033.0302242
TTACGCA3800.030.535954
ACCGTTC1902.0767402E-730.5270468
GTATTAC5000.029.012781
CGGTAAT1255.1549845E-428.9994839
GTATTAT5750.027.7513561
TACACCG3204.1836756E-1127.196085
TGTAGCG1100.00965747626.3719585
TACGGTA1100.0096672926.366467
GTTACGC3900.026.0338543
GTGTAGG4001.8189894E-1225.3861851
ACACCGC3451.07320375E-1025.225356
TATACAG6650.023.992535
GTAATAC7150.023.3290393
TACGCAG4700.023.14565
TGTTACG4750.022.9019622
TATACTG8400.022.447565
Produced by FastQC (version 0.11.5)