FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_A09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_A09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558529
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAAACCCTGGTTTACATGTGCGGCGATATGCTCAGCAACGTCGGGGC33420.1306219315864702No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT33260.12999657224913222No Hit
CTAATACATAGGCTCGTTGTAACTAAACACTTTGAAGATGTTATAAAAGA29350.11471435344293539No Hit
GGTCAGGGCGGTTGGGATACGCAAAAGAGACGTCTTGGAATTCCACCAGC28520.11147030188049462No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC28370.11088402750174026No Hit
TCTGTACTCAGTCCGTGCTCCAACTCCTGTATGAAGCCATGGGCATTAGC27580.107796315773634No Hit
GAGTACAGATGTAGGGGAGAAAGGGAAGCAGCTGGCTGCGGGACAGAGGC27100.10592023776162006No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT26860.10498219875561308No Hit
AAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGC25760.10068285331141448No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6000.043.5207631
CGAAAGC6150.042.4451753
CGGTTCG6800.040.5340461
GCGAAAG7050.037.0288122
TGGTGCG11550.035.7802128
TATACGT10750.031.7016474
GGTGCGG12900.031.4725079
TTCGCAG9100.030.2785224
GTAGAAA17000.029.8671931
GGTTCGC9800.028.1179682
GTCTAGT9950.027.701661
ACCGTGC15250.027.5745328
TCGCAGT10300.027.4548975
GTTCGCA10050.027.4169083
GCATTAA16200.026.4169241
CAAGACG21750.026.333548145
ACGATCC2001.0662487E-525.375206145
CATTAAG15650.025.0210672
CGCAGTT14700.024.1696976
TAGTACT12900.024.1696954
Produced by FastQC (version 0.11.5)