FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_A07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_A07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383466
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA32020.13434217228187859No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT29750.12481822690149555No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG26730.1121476035319992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3500.041.4451451
TACCTAT14100.039.0854457
ATACCTA15600.037.190466
CGAAAGC4300.037.103972
ACTGATC9300.036.646758
CAATTCG5400.034.906729
TATACGT5000.034.810274
CCTTATA9600.034.7498362
CTAGTAC3550.034.7285543
CATACCG1051.8546985E-434.523139
TATAAGG12250.034.336662
TAATACC17050.034.0276344
GTAATAC18300.032.8922163
ACCTATC17600.032.54888
GTAGAAA20000.031.1874711
AATACCT19050.030.8358695
GTCTAAG14750.030.4867671
CTATAAG18100.030.0534531
GTATTAC14200.029.6245251
CGTGCTA5650.029.5188878
Produced by FastQC (version 0.11.5)