FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_A03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_A03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213935
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA54220.44664664912042246No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC26770.22052251562068811No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG25620.21104919126641872No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC23640.1947385980303723No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG21520.17727473052511047No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG18620.15338547780564857No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG18180.1497609015309716No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT16920.13938143310803297No Hit
GAATCAGGAAGACGGCATCGTTGCCAACCTGTGGAGCAATGGCTTCAGCC14160.11664545465778645No Hit
GTGTATGAGCTGCAGGCCAGTCGTGTGTCCAGTGATGTTATTGATCAGAA13840.11400939918529411No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA13710.11293850164959408No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT12540.10330042382829394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3450.042.0493771
ATGAACG6400.037.387086
CGAAAGC4050.035.8080333
CTAGTAC2750.034.2780533
GAACGTC7350.033.5398648
TAGTACT4250.032.4154664
AGTGATC26000.032.3485158
CGTACTG900.003626505132.2259035
AACGTCA7900.032.1199539
GCGAAAG4600.031.5266382
TGAACGT7650.031.2767937
GTAGAAA11100.030.7130891
CATACGG1902.0751395E-730.5298025
AAGTGAT28400.030.380747
GTGATCA28700.029.8080839
ACCATCG8900.029.3279828
GCTTATG9200.029.162142
ATACGGA2252.815068E-829.003316
TACGGAG2003.2532262E-729.002127
TAGAACG1306.4794254E-427.88784
Produced by FastQC (version 0.11.5)