FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R1_A01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R1_A01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2363078
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA206270.8728869719916142No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC95600.4045571072981933No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG82540.3492902054015991No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG76010.32165675445330205No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG69840.29554674031073036No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT65760.27828112317917564No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG63220.26753243016100187No Hit
CTATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACCCTC56240.237994683205548No Hit
GGGTTACATAGAACTGATTTTTAGCATGTGCTCCAACTGAGCTTTGAGGA50950.215608625699194No Hit
GAATCAGGAAGACGGCATCGTTGCCAACCTGTGGAGCAATGGCTTCAGCC48430.20494456805911612No Hit
ACATAGAACTGATTTTTAGCATGTGCTCCAACTGAGCTTTGAGGAGATAA48360.20464834423578063No Hit
GTGTATGAGCTGCAGGCCAGTCGTGTGTCCAGTGATGTTATTGATCAGAA45680.19330720357093586No Hit
CAATAATTGGTGTCCTTTGTTAAGCGCTATTATGTGTCCTAGAAACCTAT45450.19233389672283352No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT44570.18860994008661586No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC44550.18852530470851997No Hit
AAACAAGGCATTGGGTTACATAGAACTGATTTTTAGCATGTGCTCCAACT38050.16101880682736666No Hit
CATTTATTGAGTACACTATAGATGCCAAGCATGGAATTACTTTACATACA37800.15996086460116846No Hit
CTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTTCAC37350.1580565685940117No Hit
GTTGAAGAGATTGCAGTAGTTGTAGTAGGACTCAAACCTCTGCTCCAAGG36530.1545865180920816No Hit
GTATTTCCACAAAACCGTCTCAGATTTGATTGACCAGAAGGTGTATGAGC36360.15386711737826683No Hit
GTCTTAGCCAAGTCACTTCACCACTTTTTGACTGTTTACTCATTTGCCAA34570.14629225103868768No Hit
TCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTGGAGCAAT34260.1449804026782019No Hit
GCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTGTAGTAGGACTCAAA33830.143160742049141No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC33730.1427375651586617No Hit
GTTTACTCATTTGCCAATAAAACAGTTCACATTTATTGAGTACACTATAG33390.14129876373103215No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC33200.14049472763912152No Hit
GGATAAGACTGAATGGAAACTGAACGGACAGGTGCTGGTCTTCACACTCC32790.13875970238815646No Hit
AACAAGGGGAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGC32240.13643222949052042No Hit
CCTATGAACCATGTTTAAGCATTTCACCCTCCAGAAGCCCCAGTAGCTAG32090.13579746415480148No Hit
GCTTAACAGCTATCATTTTAGGATATAAAAAGCGGTTTTCTTTTTATCTT31320.132539002098111No Hit
GCATATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGC31170.1319042367623921No Hit
AGTTGTAGTAGGACTCAAACCTCTGCTCCAAGGAAGGCCCTCCACTGACT29870.12640293718616144No Hit
GGCATATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGG29760.12593744260663423No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC28120.118997341602774No Hit
AGTAGAAACAAGGCATTGGGTTACATAGAACTGATTTTTAGCATGTGCTC28060.11874343546848644No Hit
GGATCTGGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCC27990.11844721164515094No Hit
GTGTATTCTTGAAGAACCTTTCAGTCAACTTGGTCCAGCTGTTCTCATAG27980.11840489395610301No Hit
CCACAAAACCGTCTCAGATTTGATTGACCAGAAGGTGTATGAGCTGCAGG27850.11785476399847995No Hit
CCACTGACTTTGGCATATATGTGCCTGTAGTATAATTCTTTGTACAGAAT27570.11666986870513796No Hit
ATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACCCTCCA27430.11607742105846698No Hit
GTGTATGAGATCCAGGACATCTATGAGAACAGCTGGACCAAGTTGACTGA27260.1153580203446522No Hit
ATTTTTATTAGACATGAGGTCTTAGCCAAGTCACTTCACCACTTTTTGAC27240.11527338496655634No Hit
GTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTA27140.11485020807607704No Hit
GTAGAAACAAGGGGAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAG26870.11370763047178298No Hit
ACACTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTT26800.1134114066484475No Hit
CTTTTTGACTGTTTACTCATTTGCCAATAAAACAGTTCACATTTATTGAG26140.11061843917128424No Hit
GCTCCAAGGAAGGCCCTCCACTGACTTTGGCATATATGTGCCTGTAGTAT25950.1098144030793736No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG25770.10905268467651089No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT25580.10824864858460025No Hit
GGTTAAAGGTATGAGCATATCTGAACTCCTGCTGTGGAATGTGTAGCAAG25410.10752924787078547No Hit
GGTATGAGCATATCTGAACTCCTGCTGTGGAATGTGTAGCAAGTGAATTA25100.1062173995102997No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG24810.1049901865279098No Hit
CCTTTGTTAAGCGCTATTATGTGTCCTAGAAACCTATGAACCATGTTTAA24310.10287430207551339No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC24030.1016894067821714No Hit
GAAACAAGGGGAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATT23900.10113927682454832No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG23800.10071609993406905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6650.086.200691
AGTAGAA31350.071.98271
GCGAAAG8900.063.563472
ACGGGCG808.094503E-963.436096145
CGAAAGC9900.057.141713
GTAGAAA41800.054.3089072
CGGCATA858.9314017E-751.175335145
ATGAACG22350.048.6731156
GCTTATG22000.048.4615942
TTAAGAT14100.046.2912374
TAAGATG14600.044.2091945
CTTATGA24200.044.0550583
GGCATTG13700.043.929827
GAGCGAA2450.041.463621
TATACGT5800.041.2630544
GCATTGG14200.040.3370258
AGAAACA59450.039.8906674
ACCATCG28800.038.518038
GTATACG6050.038.3608673
TATCGAG1354.9628943E-737.604494
Produced by FastQC (version 0.11.5)