FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_8_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_8_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742780
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24160.3252645467029268No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC9830.13234066614609977No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGT604.9794116E-429.1698681
GTATTAA700.001225361525.002741
CGTATGC750.001837002623.3233223
ATACTGC1356.0388047E-620.733246
CGCCGTA3100.019.20212267
GTCTATC1301.0561057E-418.8482231
CGTATCA3750.017.74125570
GCCGTAT3400.017.50427868
ATCCTAT1401.86972E-417.4936716
GCATAGG2207.2977855E-817.49249332
AGGACCG1250.001767661516.7916625
GTGTAAT1906.1095143E-616.5807651
GGTCGCC3651.8189894E-1216.30755264
GTCTGGG2151.0948443E-616.2808571
GGACCGT1300.00229530916.1480056
GTGCATA1350.00294313615.5572621
CCTATGA1350.002949750515.5520242
TTATACT1807.171633E-515.5488834
TAGTACT1807.171633E-515.5488834
CATAGGC2251.7687089E-615.548882533
Produced by FastQC (version 0.11.2)