FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_7_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_7_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271354
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11520.4245376887755478No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC4280.1577275440936931No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG3680.13561620613663333No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGCG400.002410560835.0166268
GGGTGAC450.004308263331.1029137
TTACTCG450.004308263331.1029134
TATAGTG604.9820496E-429.1589785
TTTAGAG500.007210074527.992623
CGTATCA1953.092282E-1123.34872270
TCAATAC750.001832242423.3271833
CGCCGTA2056.002665E-1122.2056667
GTCCTGG800.00266601821.8692346
GTGTAGT800.002671736321.8611681
GTATAAA800.002671736321.8611681
GGTCGCC2108.185452E-1121.68095664
CCGTATC2151.1277734E-1021.17674869
TAGACAA850.003789185820.582815
ACCAATG850.003789185820.582817
GCCGTAT2353.7289283E-1019.37089568
TATCCCT1501.4929876E-518.6617474
TCGCCGT2508.4946805E-1018.21200266
TGGTCGC2551.1059456E-917.85490463
GTCGCCG2601.4315447E-917.51154365
Produced by FastQC (version 0.11.2)