FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_6_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_6_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333354
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10600.3179802852223162No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC3680.11039315562435129No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGT200.006974059652.44984
GTCTAAA553.0004562E-431.79731
CTATCCT450.004311618431.1000339
CGCCGTA1251.03682396E-1030.816867
TAGTCCT500.007236855627.9732254
ACTGAGT700.001227270624.9910988
CACTGCG700.001228345724.9873437
AAGGCAG851.3134677E-424.685965
CCGAGCT750.001832504323.32852648
CAGTCCA952.7823568E-422.0973919
GCCGTAT1604.848698E-821.8902268
CATAGGC800.00266406921.87378139
GTCTGGG800.002673378221.8606431
ATCACCC1003.9270183E-420.99882712
GTCCACG850.003799620620.5747221
TGCGCGA1055.4298394E-420.01391665
TGGTCGC1751.2495366E-720.01391663
GGTCGCC1801.6814192E-719.45797364
GTGGTCG1801.6814192E-719.45797362
CTATACA900.00528459919.43462
Produced by FastQC (version 0.11.2)