FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_5_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_5_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72770
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2670.3669094407035866No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC920.1264257248866291No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGA150.002217539469.9917456
CCCTATG150.002217539469.9917452
GATTAAC200.006910355852.5299625
TGATCGG200.006929214652.4938134
TACATAT200.006929214652.4938169
GTGCTAT200.006929214652.4938120
ACTGTGG200.006929214652.493815
TGAGTGG350.001244519340.02282710
GTGAGTG350.001244519340.0228279
GTTGAGC450.004284673431.10744317
TGTGATA500.00717081627.9966988
CGTGTAG700.001216361624.99705170
CTTACGT1905.3842086E-1022.10265570
CCTTACG2101.879016E-919.99763969
TCCTTAC2806.582013E-814.99823168
TTATCAC1656.4224337E-414.8467323
AGCCTAA1900.001864583912.8932166
CTCCTTA3355.865677E-712.53583467
TAGGCTT2459.5302606E-411.4272235
ACTCCTT3701.9478812E-611.35001366
Produced by FastQC (version 0.11.2)