FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_4_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_4_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436630
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16470.37720724641000386No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5310.12161326523601218No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4520.10352014291276368No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAC450.00432474931.0828513
TGCGACC500.00722140127.98739210
GCTGCCC1207.4365744E-826.2321668
TATTATA808.6986896E-526.2321662
CTAGACA808.705473E-526.229164
CTGGCCC700.001231020924.9801544
GAATTTA800.002673274421.8626441
GTGTAGG1003.9432495E-420.988141
GTACCGA1003.949357E-420.983336
TAATAGA1356.0520124E-620.7242764
GTCTCAG850.003799466920.5766071
CGCCGTA2705.456968E-1219.46245867
CGTATCA2757.2759576E-1219.10640170
TTACACT1107.506957E-419.0757544
GTTTATA950.00722712818.410651
CCTTTAA950.00722712818.410651
ACTATGC950.00723190818.4085398
ACAAAGG950.007241476318.4043223
GTACTAA1000.00971256117.4901161
GTAACCG1000.00971256117.4901169
Produced by FastQC (version 0.11.2)