FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_40_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_40_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772731
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21030.27215162844508634No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC15660.20265784600333103No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG10300.1332934747020632No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG500.007207294528.0012951
CGCCGTA3350.020.90597267
GTATAAA2051.4515535E-920.4887521
GCCGTAT3450.020.29868568
GTTCTTA1852.2684617E-718.9197941
TCTATGG1301.06195366E-418.8348223
CGTATCA3750.018.67721270
GGTCGCC4150.018.564764
GTATAAG1351.4111798E-418.1489891
TGGTCGC4400.017.50988663
TAAACCC1401.8741994E-417.4883464
CGTAGTA1250.001756763916.80731257
CCGTATC4250.016.47775769
GTATTAT1704.381136E-516.471351
GTATTAG1300.00228917416.1545941
GGCATAG2854.976755E-915.95428131
TCGCCGT4950.015.56434266
TAATACT1350.00295853915.5451974
GCTTAAG1605.142362E-415.31916468
ACTGAGC1605.177922E-415.3052768
Produced by FastQC (version 0.11.2)