FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_3_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_3_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321354
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11980.37279760015434693No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC3760.1170049229198952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG901.7589446E-731.0971361
CTATGAC450.00431664531.0922953
CGTATCA1650.029.7113870
ACTATGT604.9758726E-429.1671858
TATACCT604.9895013E-429.1535644
GCCGTAT1700.028.83302568
GCGTACG500.007202127528.00049811
GCCTAAT500.007218573227.9874231
CGCCGTA1850.026.49933867
TGGTCGC2300.022.83716463
CCGTATC2207.2759576E-1222.28006469
AGTCCCA800.002662727621.8753910
CTGCAGT1003.9370504E-420.9905664
TCTATGA850.003798196620.5757852
GGTCGCC2453.092282E-1120.00970564
CGGTGGT2453.092282E-1120.00970560
CTATTGG900.005282455619.435711
ATATAAA1651.5927071E-619.0823331
TAGATCT3150.018.88628253
GGCATAG950.00720530418.41851237
Produced by FastQC (version 0.11.2)