FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_39_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_39_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943312
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25480.2701121156096816No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC10370.10993181471241752No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAC1755.293259E-921.984153
TATTAAG800.002673032721.8661962
GTACTAG1154.1211708E-521.3025531
GTCTAGG1356.0271504E-620.7389951
CCCACGA1055.4666994E-419.9993816
TATAATG1751.270273E-719.9919512
TAGTACA1301.0641083E-418.830584
GTATAAT1903.008263E-718.4195021
TACTAGA1150.001010562118.253522
CTATTAC1803.6599486E-617.4985281
TCTAGTA1200.001344447517.4929582
TATACGA1000.0097115917.4929582
TCTACGC1250.001770793616.7878953
GTATTAT1704.3876888E-516.4692021
CGCCGTA3005.493348E-1016.34236567
TCTTACA1957.862609E-616.1473462
GTGTAGG1554.0567742E-415.8051221
GTATACT1350.002947730515.5542471
ATATAAG1350.00296026415.5443493
CTATACT1350.002962357415.54274
Produced by FastQC (version 0.11.2)