FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_38_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_38_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797957
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22920.2872335226083611No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC11440.14336612123209647No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG9060.11353995265409038No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA750.001834356323.3293611
GCCGTAT2900.021.72861768
CCTTACA1154.130866E-521.2953782
CGCCGTA3050.020.65999667
CGTATCA3200.019.69155970
CTTAAGA900.005265367719.45089363
GGTCGCC3300.019.09364764
TTACACT2155.6166755E-817.8983144
GTATTAC1452.4413131E-416.8936751
TGGTCGC3800.016.58444663
CCGTATC3900.016.15717569
ATAGTAC1300.002299306416.1440073
GTGTTAG1605.166333E-415.3098931
GTGTATG2101.582891E-514.9974461
GGACTCT2101.5838305E-514.9965056
ATTACAC1656.547213E-414.839443
ATATAAT2151.9771142E-514.6486671
TATATAC1450.004751483414.476662
CATTATA1450.004751483414.476662
CTGTGTA1450.004751483414.476662
Produced by FastQC (version 0.11.2)