FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_37_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_37_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005415
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC50060.4979038506487371No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG37920.377157691102679No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18290.1819149306505274No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCAAAGC10200.10145064475863201No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC2200.025.4478321
CCCTAGA1408.268378E-619.9947241
TTAAAGG2053.259447E-818.770863
GGCATAG8150.018.45495631
GTACCTA950.00722096718.4161931
GCGTGCG950.007233418518.4106949
GCATAGG8500.018.10655832
GAGTATA1551.9887071E-518.0597521
CATAGGC8250.017.80727633
GCATACG2207.299968E-817.49364350
GGCATAA5250.017.32617249
TTATACT3053.8198777E-1117.2042924
GTACTAA1452.4438536E-416.8920941
TAAGTAT1250.001768440416.7913884
GTATATA2108.603438E-716.662271
ATACTGG2351.553035E-716.372956
CCTTTAA1503.164999E-416.3290231
TATAAGC1503.171008E-416.324963
AGCATAA12100.016.20859761
TATATAG1300.00229532916.148782
Produced by FastQC (version 0.11.2)