FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_36_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_36_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813093
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19780.24326860519030416No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC13650.16787747527035654No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG10310.12679976337270152No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3100.023.7237267
GTATAAG1356.0158945E-620.7428041
CGTATCA3500.020.00814870
GTACACT2353.8198777E-1019.350524
TATAGAG1301.0618472E-418.835275
GCCGTAT3750.018.67427368
GTATAGT950.00720469518.4228821
CTTAGGA950.00722776118.4126782
GTGTAGG1551.9817417E-518.066311
GTGGTCG4300.017.9164862
GGTCGCC3950.017.73092364
TGGTCGC4000.017.50928763
GACCGTG1401.8739658E-417.4888157
CCGTATC4500.016.33898269
CCAATAC2452.4863766E-715.7081093
TAGGACA1350.00295697315.5465724
ATCTCGG5000.015.40247956
GGTGGTC4850.015.16268361
TATAAGA1859.064218E-515.1282552
ATACGAC1400.003762485714.9941043
Produced by FastQC (version 0.11.2)