FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_35_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_35_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658903
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17670.26817300877367384No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC9970.151312105120177No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6730.10213946514130304No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTATA500.007218332527.9919171
CTATATG750.001835282523.3265971
GGCATAA1751.2581768E-720.00790843
GTATAAG1408.263045E-619.9942261
GCGGCCC900.00526247319.45213123
GGCCTCA900.005283328219.4388312
CGCGTGG900.005287972319.43587710
ATGTAAG1651.5954465E-619.0853961
CCTAGAG1107.4984966E-419.0810472
TATAAGA1651.5989317E-619.0810472
GTATAGG950.00721942618.4157331
TTACGGC950.007238435618.4073433
TTAATAT1000.00972770917.4869753
ATAGAAC1803.6796591E-617.4869753
CAGGACT1653.416094E-516.9557784
TAACACA1452.452645E-416.8826944
GGCATAG2351.5543264E-716.36954131
ACCGCCA1503.1488936E-416.33854917
GTTTATA1503.163523E-416.3286171
TTAAGAT1300.00227988716.1639270
Produced by FastQC (version 0.11.2)