FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_34_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_34_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979777
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26530.2707759010468708No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC11960.12206859315946385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3850.026.37970567
CTCTAAG700.001230848424.9845621
CGTATCA4400.021.48816370
GCCGTAT4800.019.69748568
TGGTCGC4850.019.49541563
GGTCGCC5050.018.72331864
CCGTATC5350.017.6725169
CGGTGGT5600.017.50887560
ATACCGC1200.001346897817.4883026
CCATAGG1200.001348743717.4847283
CTCGGTG5650.017.35392858
GGTGGTC5650.017.35392861
TAGATCT5650.017.34771753
GTGGTCG5900.017.21211262
ATATAAA3700.017.0165141
ATACTAT1854.7786434E-617.0156426
GTGCTAT1452.4503813E-416.8861181
TAAGGCA1452.4551642E-416.8818054
ACACAGG1250.001770242916.7887696
TATATAA1755.656664E-515.9876692
Produced by FastQC (version 0.11.2)