FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_32_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_32_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936649
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16070.17156907229922844No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC12820.136870908953087No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGGT952.7934325E-422.090544
TATTATA2051.4624675E-920.4774382
GTATTAG1205.714994E-520.4172421
CGTATCA3150.020.0059170
TATAGGC1107.506585E-419.0792123
GTGTTAG1852.2701715E-718.9194551
GTATACG1301.05714E-418.8466851
CGCCGTA3400.018.5358867
GTACTAA1150.001007636218.2613851
GTACTAG1000.00968748917.5004941
GGTCGCC3650.017.26629664
GTACTAT1957.830391E-616.1543031
CCTCTAG1755.6177087E-516.0004521
GCCGTAT3950.015.95493468
CTACACG1554.0747487E-415.7959224
CATAGGC3151.1095835E-915.55183927
TGTTAGG1807.179544E-515.5476862
ATATTAC2051.2582079E-515.3662871
CCTAGAG1605.180725E-415.3047532
GTATATG1400.003752519615.0004241
Produced by FastQC (version 0.11.2)