FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_31_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_31_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785930
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC28000.35626582520071765No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG21890.27852353263013246No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10480.13334520886084003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC1502.4794645E-823.330131
CGGGGGC952.784103E-422.10081936
TATAGAC952.7889028E-422.0951922
GGGCTAC800.002668528421.8719961
ATACAGC1304.359197E-621.528656
TAGAGCG2151.1641532E-1021.153157
AGAGCGC2501.8189894E-1220.991778
TAATACG1003.9442157E-420.9904334
GTATATC1257.830594E-519.5973071
TTAGACA1257.849773E-519.5910724
CTAGGGT1107.4971834E-419.0822124
GATACCG1301.0607962E-418.8375685
CTATACT1301.0607962E-418.8375684
GTATAGC950.007214334318.4185221
AGCGTAC950.007222288318.41500510
CGTTATA950.00722759518.412662
GTATTAG1150.001008560318.2583621
GTACTAA1150.001008560318.2583621
TAGGAGT1150.001010718318.2525524
TTAAGCA1401.8630543E-417.50205446
Produced by FastQC (version 0.11.2)