FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_30_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_30_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010418
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC14930.14776062975916898No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13770.1362802325374251No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG10230.10124522722279294No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATTA1751.2748751E-719.9854453
CGCCGTA2659.276846E-1118.50367567
TAGGAGT1150.001013177418.2466774
GCTTAAG1200.001339446417.50286144
TACTCTG2259.479663E-817.097815
TTATACT2056.722894E-717.05994
TAGACCC1653.417751E-516.9565094
GTGCTAG1452.4313039E-416.9035021
TAATAGG2552.1642336E-816.4773651
TAGGTTG1704.4143297E-516.4577875
GTATTAA1503.1487725E-416.3400521
GTATATA1300.00228425516.1604921
GTCTAAT1755.6000335E-516.0065821
TTAGGGT2201.405775E-615.8967274
TACGGCT3108.8039087E-1015.80903640
CTAAGAC1350.002936264515.56349857
TATATTG1807.1971066E-515.5434655
GTATTAT2503.1009404E-715.4063351
GGGAATA13500.015.30107220
TATTATG2302.2195218E-615.2146032
Produced by FastQC (version 0.11.2)