FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_2_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_2_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339756
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12850.37821259963032294No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5000.14716443565382215No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATAG400.00241299235.01194823
ATTAGGG450.004321913731.0850473
GTATACT450.004321913731.0850474
GTGTTAA500.007227649427.9806651
CGTATCA1900.025.80589370
CGCCGTA2050.025.6222867
GTCTACA851.3125052E-424.6888221
GCCGTAT2001.8189894E-1224.51559868
GGTCGCC2259.094947E-1221.79164164
TGGTCGC2351.6370905E-1120.86433663
TTGGACT1408.282921E-619.9832464
ATAGCTC900.00529494819.4281548
GTTCATA950.00722913918.4083351
TACAGAG950.007235288618.4056225
CCTGGTC1150.001011703218.2455713
TGGACTT1351.4178018E-418.1329465
TCGCCGT2952.1827873E-1117.80531166
CGGTGGT2802.0372681E-1017.5111460
GTGGTCG3002.910383E-1117.5111462
CTTTTAT2207.2850526E-817.4879151
Produced by FastQC (version 0.11.2)