FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_29_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_29_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401044
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8650.21568705678179947No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5720.14262774159443853No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATGT200.006956787352.4846538
GGTTTAG700.00122368325.005171
GTGTTAG750.001828753923.3381611
GTGCGCG952.782526E-422.09889
TATACTT952.7895812E-422.0905325
AGGGTGT800.002672540321.863156
TTATACT800.002674476721.8604224
TATGAGT850.003801174220.5745144
CTAACAC850.003801174220.5745143
TGTAAGC850.003801174220.5745143
GCAGTGT1205.7296475E-520.4056056
GGGGTTA1055.478001E-419.9891646
CAATACT1055.482609E-419.9866734
CGCCGTA1801.6855847E-719.45575367
AGTAGCG950.0072303718.4087775
TCGCCGT2601.4388206E-917.5101866
CTGTCGG1000.00968902817.49706811
CCTGGAC1000.00971691117.4883373
CCACACC1000.00971691117.4883374
GCCGTAT2056.6120083E-717.08096768
Produced by FastQC (version 0.11.2)