FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_28_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_28_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436780
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7730.17697696780988142No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5090.11653463986446266No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC500.007224801527.98473
TAGAGTG808.6976084E-526.2326535
TACCACG700.001230067624.983487
GGATAGA851.3049376E-424.7121931
GTATATA851.3049376E-424.7121931
GGTCGCC2400.023.34464364
GTTAGGG750.001837050823.3205833
GTGTATA1154.103891E-521.309791
TATTACA1003.9366723E-420.9933342
GCCGTAT2750.020.36883768
CGTATCA2800.020.0051170
GGCATAA1257.796849E-519.60500543
GTGTATG900.005263943719.4494111
GTAAGGA900.005263943719.44941142
ATCCCAC900.005274418319.44272615
ATAGTGT900.005291913619.4315956
GCGATAA1107.4541406E-419.09578540
TAGGGTG1107.500249E-419.078295
CGCCGTA2951.8189894E-1218.99225467
TCTAGAC950.00722636718.4109863
Produced by FastQC (version 0.11.2)