FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_27_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_27_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399447
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5770.1444497017126177No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5190.1299296277103095No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGT459.191779E-538.88841
TAGGGTG459.218966E-538.868915
GTATTAT552.9901968E-431.8177831
GACCCCG500.007214079627.99263211
TTAATAC657.974083E-426.9092463
TTATAGA750.00183374923.3271942
AACACTA952.786669E-422.0939085
CATCTAA850.00378676620.5879781
ATAGTGT4300.020.36134170
TTATAAC1055.458432E-419.9997481
CACGACC1150.001005685218.2629343
ATCCTGG1000.00968031217.499781
CTGTCCT1000.00970129217.4932029
ACTGTCC1200.001342480217.4932028
GGACCGT1200.001342480217.4932026
TTTACAC1602.6120151E-517.491013
GGGTTGG1250.001760020416.799791
TAATACA1250.001765933716.791374
TACACTG1503.16504E-416.3249445
AGAGCGC1755.6247838E-515.9937858
Produced by FastQC (version 0.11.2)