FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_26_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_26_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747424
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11780.15760799760243183No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC11190.14971421843558674No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG7610.10181637196557777No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTATA1705.456968E-1226.7510172
GCCTAGG800.002667983821.8725951
TTAACCA1003.950809E-420.9850464
GGCATAG1709.366704E-820.57632831
CGTATCA2950.020.17943270
GTGTAGC1055.468148E-419.9978031
CTATACT1055.4903945E-419.9857584
CGCCGTA3000.019.84443967
ACTATAC1150.001011944418.2490863
GTATTAT2155.5913915E-817.9050061
GCCGTAT3350.017.76875768
GTTATAT1000.00969342317.4980751
GTATAAG1401.8661424E-417.4980741
TATAACC1000.009715863517.4910492
TAGGACA1000.009727117.487544
CATAGGC1854.7745343E-617.0160433
CTAATAC1250.001769438616.7891583
TATACTT1250.001770230916.7880365
GTGTATA1704.3866967E-516.4687771
TAAGCCA2803.8926373E-916.25256540
Produced by FastQC (version 0.11.2)