FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_24_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_24_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563093
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16940.3008384050236817No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAC200.00692727552.54387359
GTATATG755.6054905E-527.9835341
ATATACC952.7967585E-422.0844084
CCTACCC800.002677350321.8582483
CGCCGTA1601.173752E-619.70570867
CCCATGC900.005291543419.4330089
TAGGTGG900.005302433419.4261025
GTCTTTA1107.500248E-419.0796811
GTATAAA1903.0188494E-718.410221
GTAATAC1150.001011132318.2501321
GTACAGG1351.417188E-418.1374761
GGTCGCC2005.0846575E-717.51774464
TCTTAGA1000.00971725317.4897082
GCCATAT1200.001345207117.4897081
TATATAC1000.00972720617.4865993
CTATTGT1000.009737167517.483494
GGCATAG1452.4541572E-416.88061131
TGGTCGC2301.1917655E-716.7546163
AGAACAG4050.016.4042635
GTGTTAT1503.1697247E-416.3237291
Produced by FastQC (version 0.11.2)