FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_23_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_23_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562524
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21720.38611685901401543No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC8330.14808257069920572No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG7080.12586129658467904No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCAC604.9837196E-429.16403442
ATTAAGA952.7856795E-422.0973662
TACACAG1205.7385358E-520.4039295
GCCGTAT2453.092282E-1120.01244468
CGCCGTA2302.8194336E-1019.79315667
GTAACAT900.00529808619.4288543
CTAGACT950.007232127218.409564
CTCCGGT950.00723955418.4062823
TAATACT1200.001345529117.489084
CTAAAGA1200.001345529117.489084
CTTACAC1200.001347134617.485973
CGTATCA2651.8608262E-917.17896370
TCCTGGG1452.4431868E-416.8905162
AGTAACA1250.001765294516.7939992
GTGTATA1503.1662913E-416.3260461
TATACAG1350.002951116315.5499992
GCTCTAA1350.002952867215.54861451
GTAACAA1807.1677234E-515.54861451
CCGTATC3201.366061E-915.32066469
GGTCGCC3009.622454E-915.17610464
Produced by FastQC (version 0.11.2)