FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_22_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_22_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494596
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12260.24787907706491763No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC9030.1825732517044214No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG7430.15022361685092478No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT400.002427433634.972534
CTAGGCA500.00723412727.9780244
GTATATA700.001225388425.0006141
TAGACAT1003.9501378E-420.9835195
GTGTAAA1205.708154E-520.417171
TAATAGG1558.726438E-720.323081
GTTCTAT1257.813355E-519.6004831
TGCACCG1107.508427E-419.0759265
TGCTAGG950.007216537418.4159132
TAAAGAT1351.4035602E-418.15962870
CGTAGTT1401.8741809E-417.4862655
GCTCGCT7900.016.83926821
CTGGATA7550.016.68408630
CTTATTC2108.6194996E-716.6552723
GCCAAAG3005.493348E-1016.3353943
GTATATG1300.002287936416.1542431
AGACTAA1300.002289484316.15260954
ACCTATC3950.015.9384633
CACCTAT3753.6379788E-1215.8622372
CATAAGC2009.928706E-615.75198345
Produced by FastQC (version 0.11.2)