FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_21_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_21_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326583
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13430.41122777364406593No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC3320.10165869013390164No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGA305.8847904E-446.647982
TTAACTA450.0042962931.12248644
TTATATA604.988252E-429.1549872
TATAGAA500.00721702127.988792
GTATATC700.001225345524.9976461
ATATACT700.00122973124.9823364
GACTGCC901.925986E-423.33113711
TACTACT901.9340015E-423.3168474
TAATAAC800.00267167121.8628943
TCTTACA1003.9359415E-420.9915922
TAGGCAT1501.5002357E-518.65347929
TACTGTT950.00722924318.4080377
TGATCCG1351.416169E-418.1353265
TTAGGCA1000.00970684617.49031428
TAGTGTT1000.00971540917.4876374
TATCCAG1452.4457654E-416.8846155
AGGCATA1704.3969612E-516.45895230
CTGGACA1350.002954449315.5445654
GGACAGA1450.004752524714.4725276
GTCTTCA2001.749066E-413.9986821
Produced by FastQC (version 0.11.2)