FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_20_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_20_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437758
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13620.311130807432417No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5670.12952361807208548No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4750.10850744018384587No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG604.99566E-429.1503355
TATAGCA500.00721320627.993922
GGACGTG700.001227710324.99171411
GCATAAC700.001229347524.9860023
TAGGGGT700.001229347524.9860024
GTTATAG750.001829883923.336271
TATACTC800.002673210821.8627515
CCTTTAA850.003784306820.5908241
GTCGATA1055.446096E-420.00709221
GTATTGT900.005267908319.4468921
GTGGTCC950.007212672418.41705126
CAATGCG950.00722219318.4128429
TAGAGCG1150.00101045318.2506437
GCCGGAG1000.00966131517.50620722
TACTCCG1200.001344306317.4902025
GTAGTCC1000.00971232917.4902028
GACAGTG1704.3969383E-516.4613657
CGCCGTA1503.139543E-416.34286367
AAGGGTG1503.1670308E-416.3241885
TTGTACT2201.3981589E-615.9001834
Produced by FastQC (version 0.11.2)