FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_19_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_19_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305405
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8320.2724251403873545No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC4880.15978782272719833No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG3220.10543376827491363No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACT501.7115225E-434.9861452
GTATAGT450.004301520531.1141031
TCAATAC605.001739E-429.1407893
GGGTCCC700.001229667224.9819097
TGTACTC750.001837680823.3164525
TCTATTC1202.1769993E-623.3126323
ACAGGAC800.002676458821.855593
TAAATAC800.002676458821.855593
TGTAGAT2705.456968E-1219.45908270
GGGAATA900.00525025519.45588720
ATCTATT900.0052702219.44312325
GTATAAA1107.4550783E-419.0927451
AGGCATA950.007215222318.41376136
TAGGCAT950.007215222318.41376135
ATAGCGA950.00722888718.4077246
CATAAAT1200.001337144617.5016821
CTGTAGT1200.001344515217.4873394
GTGTAGA3650.017.27326670
AGCAGTC1250.001767126316.7878467
TAGTGTA3800.016.58870368
Produced by FastQC (version 0.11.2)