FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_18_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_18_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296532
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8640.29136821658370765No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC4960.16726693914990626No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG3850.1298342168804716No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGGC450.00431229731.09791433
TAATAGA604.992779E-429.1493784
GGTAGCA500.00721086227.99284612
TAATATA500.00722275127.9834024
CCGTTCA906.1756527E-627.2106749
TTAGGCA750.001834359423.32343728
TAGAGGT800.002669089521.8657237
GGCATAG800.002669089521.86572331
ATATTGC900.005265311319.446041
ACCGTTC900.005280747519.4361978
CACGATT950.00720891218.41634626
AGTGCCA950.007215934318.4132410
CTCGCCT950.007215934318.4132428
AGGCATA1351.4125502E-418.1404530
TAGGCAT1452.4395394E-416.88938529
GTTTTAC1300.00228415816.155171
CGTATCA1350.002924897515.56734170
TTATTGC1400.003759977914.9911084
GAATTGG1901.1286212E-414.738051
AGAGAGG7950.014.53930970
Produced by FastQC (version 0.11.2)