FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_16_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_16_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050997
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38120.362703223700924No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC12840.12216971123609296No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG959.440295E-625.7741457
GTATTAA1356.0349885E-620.7363111
CGCCGTA4450.019.67277567
GTATACA1651.5961468E-619.086831
ATACTAT1752.8129089E-617.9884456
CCCCTAC1602.611856E-517.4962621
TATAGTA1000.00972038917.490432
CCTACAC1653.4139954E-516.9588033
GCCGTAT5250.016.67422568
CGTATCA5300.016.51613270
GTATTGA3251.0004442E-1016.1503961
TTAGTAC1300.002300463216.1434763
GTTCTAG3057.021299E-1016.0621411
GTCTAGG2009.982114E-615.7466351
AGAGGCC2001.0009198E-515.7421378
GATATAA2051.2613857E-515.3625711
CTCTAGG1605.183084E-415.3041272
TATACGT1605.1868346E-415.302674
TAAGCAT5950.015.30178159
CCGTATC5950.015.30105269
Produced by FastQC (version 0.11.2)