FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_15_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_15_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547885
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21530.39296567710377184No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC9600.17521925221533716No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG7180.13104939905272092No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAG350.001253727240.0095021
CTATGCG500.007224496527.9861959
ACCGGTC700.001229263624.9876738
GTCCTAG750.001829279523.3388771
AGGCATA2504.0017767E-1119.60286342
CGCCGTA3200.018.60666867
CGTATCA3200.018.60496770
GCCGTAT3400.018.537268
GTCTAGG950.007196027318.425431
GTATAGT1351.4081654E-418.152461
TGAATAG1551.9915093E-518.053965
TACATCG1000.00971672617.4897755
AGACTAA1452.4251931E-416.90674460
CCGTATC3750.016.80706469
TATAATA1250.001766823516.7917162
GGTCGCC3651.8189894E-1216.31269664
ATCTAGA2201.3881327E-615.912871
TAGGCAT2906.184564E-915.69194841
GTATTGA1350.00293930915.5592511
TAGAGCG1807.1700626E-515.5478867
Produced by FastQC (version 0.11.2)