FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_14_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_14_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210509
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7980.37908117942700786No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG200.00693112252.5249821
GTATATG551.3008867E-744.566651
TATATGA501.7081299E-434.991682
TACACCG552.9935452E-431.8030535
GGTCGTT450.004305544331.10371616
GTAACCT450.004305544331.1037164
GTCCAAT500.00718048228.0133271
AGTCGGC750.001830608723.32778512
ATACTCT750.001833143523.322246
AGATGTG1154.114027E-521.294227
CTTGTCT900.005277313819.4352029
AAGGGAG900.005277313819.4352025
GTTGGAT950.00720138718.4166723
ATACTTT950.00721127518.4122966
GATCAAA1200.001331878617.5083271
TTCTATT1000.00967808517.495842
ATAAAAG1000.00967808517.495843
ATCCCTT1000.00969134617.4916826
GTGTGTA3053.6379788E-1117.22130670
TGCTCGC1554.0501627E-415.79893710
Produced by FastQC (version 0.11.2)