FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_13_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_13_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332472
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10180.30619119805577616No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA450.00431012331.1022131
ATATTAG450.00431012331.1022131
GTTATAT755.587166E-527.991991
TTGTATA500.00722378327.9835622
TCTAAGG500.00722378327.9835622
GTATTAG657.9606514E-426.9153751
TGACGTT959.43644E-625.7665795
GTCCAAG700.001226762924.9928471
CCGTGCA700.001227840324.9890869
TGGCTTA700.001228918624.9853256
TACACTG901.9364792E-423.312625
GTATAAA850.003791454520.5823461
TACATTG1055.4880866E-419.9822445
GCCGTAT1257.777015E-519.60915668
GGCATCC900.00528244119.4359558
AGTAAAG900.00528704119.4330312
ATACACT900.005296251319.4271834
GTACCAT1107.491358E-419.0797046
CGCCGTA1301.0497705E-418.85779867
ATTCTAG1501.494933E-518.6613271
Produced by FastQC (version 0.11.2)