FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_12_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_12_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564108
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18690.3313195345572124No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC7630.13525778751586576No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6890.12213973210803605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA3250.024.78525570
CGCCGTA3500.022.01618867
TTAGTGC800.002673333321.8639138
GCCGTAT3550.021.70224868
TCTATCC1055.480507E-419.9898643
GGTCGCC3900.019.75636564
CCGTATC4000.019.26245569
TAGACAG1150.001011186918.2499945
AACTGCG1551.9903497E-518.055367
TGGTCGC4350.017.71260363
GTATTCG1803.6508536E-617.5004431
ATAGTAC1000.00971273217.491133
TAAAGTG1200.001345279217.489585
TAAGTAA1000.00971769417.4895785
TATACTG1401.8720579E-417.4895785
CCTAAAC1250.00176707316.7914853
TATGACA1704.401902E-516.460784
GTCGCCG4900.016.4406665
TAGTACT1503.1699223E-416.3236064
CGGTGGT4750.016.21957460
Produced by FastQC (version 0.11.2)