FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_11_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_11_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344740
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13510.3918895399431455No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC4140.12009050298775889No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAC350.001256516839.98721751
CTTATAT500.007235201527.9747983
CTTACAC500.007235201527.9747983
GTAGTAC700.001231256424.97753
TGTATAA750.001838496923.3157182
CCTAATA750.001838496923.3157182
TATAAGA800.00267509421.8584862
CATAGGC850.003779716820.5936245
ACTAGAG950.007238106318.4044728
GCATAGG1000.0096624317.50457644
ATATTTA1000.00969481917.4944081
TTGCACC1000.009735431517.4817124
TAAGAGT1000.009735431517.4817124
TAATGAG1250.001771561916.7824445
ATTCCGT1300.002302442216.1369656
TATTCCG1300.002302442216.1369655
TGTCCGA1554.0591968E-415.79910610
GACGGTG1350.002958705415.5415567
TAGAAGT1605.190149E-415.2964994
TGATTAC1450.00474559114.47602810
Produced by FastQC (version 0.11.2)