FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_sp29_10_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_sp29_10_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557441
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14200.25473547873227836No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC6420.11516913897614275No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT750.001838833823.3180776
GCTAAAG850.00377513320.60065568
GTATTTA850.00379285520.584011
TAAGCAG1558.795214E-720.3074725
AATAGGG1257.84027E-519.5924592
TTAAACG900.00529624119.4299894
ACCTCGC1107.4989174E-419.080148
TACACTT2053.2603566E-818.7665255
GTTATAT950.007214587618.4172731
CCCCCAC1150.001008518618.2571241
GTCCCAA1401.8664262E-417.4964081
CCTAGAG1000.00970576117.4932672
CCTCGCA1000.009710778517.4916979
TTACACT1401.8741292E-417.486994
TAAGCCA1854.725858E-617.0327146
TCCTAGT1250.001765593816.7935372
CCCCACA1350.002951614615.5495712
CTAAGCA1350.002958685915.5439914
AGTGTTG1859.064542E-515.1265978
CTGTACT1400.00376929214.9888484
Produced by FastQC (version 0.11.2)