FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2592_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2592_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398257
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17830.4477008564821208No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC10790.2709305800023603No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG7270.18254544176248No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTC400.00241830534.99773834
ATAGCGC400.002419796834.993348
CTATAGT604.984054E-429.1611184
GCTTAAG959.354977E-625.79753368
TATACAG700.00122650524.9952435
AGGGCTC700.00122650524.9952435
TATTAAG851.309726E-424.6980762
TATAGGA1001.3991974E-524.4922582
GTATTGG1253.0718911E-622.4098191
CGTATCA2401.8189894E-1221.88183870
ACTATAG800.002669004521.8680883
CCAAAGC1957.767085E-1021.5343632
TATAGTG1003.9324485E-420.9960025
CGCCGTA2351.6370905E-1120.86020367
TAAAGCA900.005276724719.4407444
TATATCG900.005276724719.4407445
GGTCGCC2757.2759576E-1219.09447764
TAGAACA1651.5907099E-619.0872764
GTAGAAC1107.481266E-419.0848773
GCCGTAT2607.094059E-1118.85204568
Produced by FastQC (version 0.11.2)