FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2591_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2591_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788588
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34270.43457420097693605No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC19770.250701253379458No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG17140.21735050495315678No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA500.007207353628.0013221
CGTATCA4250.027.19155970
CGCCGTA4350.026.56984167
GCCGTAT5000.023.11282768
TAGGACC952.7914008E-422.0922814
GGTCGCC5500.021.64837664
AGTAAGG1003.9392943E-420.9943282
GTCGCCG5950.020.01237565
TGGTCGC6200.019.7702963
TCGCCGT5900.019.58838566
CTGTCCG1107.4846693E-419.0869649
TTATACT3400.018.5185324
CTGGGCT1351.4180946E-418.137494
GTGGTCG6800.017.50971862
CCGTATC6650.017.37806569
TAGTATA1452.4490553E-416.886634
ACAGTGC2151.0994045E-616.2746728
CTAAGAC1300.002288246816.15563457
ATACTAG1300.00228921716.1546081
ATAGGAG1755.6424942E-515.9916185
Produced by FastQC (version 0.11.2)