FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2586_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2586_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394841
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11550.2925228129804149No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC6230.1577850324561026No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG5280.13372471450533252No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGGC450.004305445631.1105516
CGCCGTA1103.1592208E-828.6503767
TATACGT657.974444E-426.9089472
TATAGTG808.6800006E-526.2395485
CGTATCA1202.1561373E-623.34178570
GCCGTAT1202.1561373E-623.34178568
TTAATAC901.9326138E-423.3210893
CTAACAC750.001836508423.3210893
CTAGGAC750.001836508423.3210893
TAGGACA952.7868032E-422.0936624
GGTCGCC1355.9827653E-620.75088764
TGGACTG1055.472498E-419.9920355
TATACTA1055.472498E-419.9920355
CCTAATA1257.841326E-519.5897122
GTATTAC1257.848965E-519.5872331
GTGGTCG1801.6870581E-719.45395762
AAGACCG900.005282995819.4367035
GTCTATC900.00529074119.431781
TCTAATG1451.1191891E-519.300213
ACTAAGA1107.491638E-419.080892
Produced by FastQC (version 0.11.2)